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Figure 3 | Molecular Cancer

Figure 3

From: Receptor protein tyrosine phosphatase beta/zeta is a functional binding partner for vascular endothelial growth factor

Figure 3

RPTPβ/ζ regulates VEGF 165 -induced VEGFR2-α ν β 3 interaction. Down-regulation of RPTPβ/ζ expression by siRNA was followed by treatment of serum-starved HUVEC with VEGF165 (10 ng/ml) for 10 min. (A) Cells lysates were immunoprecipitated for β3 and analyzed by Western blot for the presence of VEGFR2 and β3. (B) Formation of β3-VEGFR2 complexes as evidenced by in situ PLA. The box plots indicate the median, mean and range of the detected signals (n = 8 image fields with ~4 cells per image per sample type, each sample run in duplicate). Scale bar corresponds to 10 μm. siNeg, HUVEC transfected with a negative control siRNA; siRPTPβ/ζ, HUVEC transfected with siRPTPβ/ζ#1.

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