Figure 3

NTS/NTSR1 promoted the proliferation of glioma cells. A, The expression of NTSR1 in GL261 and U87 glioma cells were detected by immunofluorescence staining. B, CCK8 were used to test the effect of NTS, SR48692 and NTSR1-siRNA on the proliferation of GL261 and U87 glioma cells. C, Immunofluorescence staining showed BrdU-positive GL261 and U87 glioma cells in the different groups after BrdU incorporation. D, The histogram of BrdU incorporation experiments. *p < 0.01 vs. the control group using a two-tailed t test, **p < 0.01 vs. the control + sc-siRNA group using a two-tailed t test, ^# p < 0.01 vs. the NTS + sc-siRNA group using a two-tailed t test. A, B Merged images; the nuclei were counterstained with DAPI and are shown in blue. Laser confocal scanning microscopy, scale bar = 20 μm.