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Figure 2 | Molecular Cancer

Figure 2

From: β-Catenin-Gli1 interaction regulates proliferation and tumor growth in medulloblastoma

Figure 2

Gli1 proteasomal degradation is partly reduced with MG-132. (a) Ptch+/- MB cells were treated with 10 mM NaCl or LiCl for 24 h. 10 μM of proteasome inhibitor MG-132 (A. G. Scientific) or DMSO was added 4.5 h before lysing cells for Western blot analysis. Membranes were probed against Gli1 (R&D), β-catenin (BD Transduction Laboratories, San Jose, CA, USA) and α-tubulin (Sigma-Aldrich). (b) Bars represent mean ± s.d. for Gli1 level after 24 h of NaCl or LiCl treatment and 4.5 h treatment with DMSO (grey) or MG-132 (black). The decrease of Gli1 protein levels under LiCl treatment was partly reversed by proteasomal inhibition (n = 2, Analysis done in GraphPad Prism 5.01 software).

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