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Figure 1 | Molecular Cancer

Figure 1

From: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

Figure 1

The expression of Notch1 and Jagged1 in human breast cancer cell lines. (A) The protein expression of N1ICD in a panel of breast cancer cells (MDA-MB-231, T47D, MCF-7, ZR-75-1, and SK-BR-3), HMECs, and MCF-10A cells was evaluated by western blot. Protein samples (150 μg) were separated by 10% SDS-PAGE. β-Actin was used as a loading control. (B) The mRNA expression of Notch1 and Jagged1 was estimated by real-time PCR. GAPDH was used as a normalization control for quantifying the expression of each target gene. Experiments were performed three times. Column: mean; bar: SD.

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