Figure 4From: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner The effects of Notch1 knockdown and Jagged1 on breast cancer EMT. (A) Nuclear protein was extracted for western blot analysis, and a β-catenin antibody was used to observe the changes of β-catenin in the nucleus. LaminB1 was used as a loading control for nuclear lysate. (B) Immunofluorescence analysis of E-cadherin and vimentin in MDA-MB-231 cells. DAPI staining was used to detect nuclei and is merged with E-cadherin and vimentin in their respective panels. The cells were observed under an immunofluorescence microscope at 400× magnification. The data are from three independent experiments.Back to article page