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Figure 8 | Molecular Cancer

Figure 8

From: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

Figure 8

Slug serves as a mediator for Notch1-induced EMT, migration, and invasion. (A) and (B) Western blot analysis and real-time PCR assays were carried out to evaluate the expression of Slug when MDA-MB-231 cells were transfected with negative control siRNA (siNC) or Slug siRNA (siSlug) for 48 h. (C) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. E-cadherin and vimentin protein levels were evaluated by western blot. (D) and (E) MDA-MB-231 cells were transfected with siNC or siSlug for 48 h and then treated with Jagged1 for an additional 48 h. The cells were seeded into a migration chamber or a Matrigel-coated invasion chamber and incubated for 24 h. The number of migrated cells was counted under a light microscope. (F) MDA-MB-231-shNC or MDA-MB-231-shNotch1 cells were transfected with negative control vector (pcDNA3.1) or Slug overexpression vector (pcDNA3.1-Slug), respectively. Forty-eight hours later, western blot analysis was performed to assess the expression levels of E-cadherin and vimentin. (G) and (H) MDA-MB-231-shNotch1 cells were transfected with pcDNA3.1 or pcDNA3.1-Slug for 48 h and then the migration and invasion abilities were evaluated. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P < 0.05).

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