Figure 2
From: Deregulated microRNAs in triple-negative breast cancer revealed by deep sequencing
RT-PCR validations of six differentially expressed miRNAs in the miR-532-502 cluster in triple-negative breast cancer. (A) A conceptual diagram illustrates the genomic loci harboring the 5p miRNA forms (brown) and 3p forms (orange) in the miR-532-502 cluster. The names of specific miRNA forms are provided under each locus. Relative expression levels of (B) miR-532-5p, (C) miR-188-3p, (D) miR-362-5p, (E) miR-501-3p, (F) miR-660-3p, and (G) miR-502-5p in triple-negative breast cancer tissues (n = 19) and adjacent normal tissues (n = 4) are shown. In each panel, paired tissue samples from the same patient are indicated with the same digits in the sample ID. The miRNA expression level in 557-N served as the calibrator control. All six miRNAs examined in the miR-532-502 cluster were markedly up-regulated in triple-negative breast cancer. Each bar represents the mean of triplicate measurements ± the SD.