Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: Male-specific hepatitis B virus large surface protein variant W4P potentiates tumorigenicity and induces gender disparity

Figure 2

Transforming activity of W4P variant and gender disparity in tumorigenesis of W4P LHB expressing. (A) Induction of cell transformation by LHBs. NIH3T3 cells and NIH3T3 cell lines expressing WT and W4P LHBs were subjected to a colony formation assay. Data represent means ± SD from three independent experiments. **P < 0.01 versus pIRES2-WT, ***P < 0.001 versus NIH3T3 cell line, one-way t test. (B) Tumor-forming abilities of LHB-expressing cells. Nude mice were injected with NIH3T3 (male, n = 10), WT-LHB-NIH3T3 (WT) (male, n = 10) and W4P-LHB-NIH3T3 [male (n = 10) and female (n = 10)] cells. The graph shows the tumor incidence of each group. (C) Comparison between male and female mice in terms of tumor size in nude mice injected with W4P cells. *P < 0.05. (D) Hematoxylin and eosin staining of the tumor mass of nude mice injected with W4P cells. Expression of LHB was confirmed by immunohistochemical staining using preS1 Ab (AP1).

Back to article page

Molecular Cancer

ISSN: 1476-4598