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Figure 5 | Molecular Cancer

Figure 5

From: Male-specific hepatitis B virus large surface protein variant W4P potentiates tumorigenicity and induces gender disparity

Figure 5

Effect of estrogen on W4P-LHB-mediated IL-6 production and cell proliferation. (A) Cells were incubated for 48 h with or without 20 nM estradiol. Production of IL-6 was determined by ELISA. (B) J774A.1 cells were co-cultivated with indicated cells for 48 h in the presence or absence of 20 nM estradiol. (C) J774A.1 cells were treated with 10-mg lysates made from tumors expressing W4P LHB in the presence or absence of 20 nM estradiol. (D) Cells were treated with vehicle (0.01% ethanol) or 10 nM β-estradiol and subjected to MTT assays. (E) Cells were treated with vehicle (0.01% ethanol) or 10 nM β-estradiol for 48 h and analyzed by immunoblotting to determine the level of stat3 phosphorylation and cyclin D. Data represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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Molecular Cancer

ISSN: 1476-4598