IRF5 inhibits wound healing and matrigel evasion in MDA-MB-231 cells. A) MDA-MB-231 cells were retrovirally infected with either empty vector (EV-231) or IRF5 (IRF5-231) expressing pBabe plasmid. Levels of IRF5 and GAPDH protein expression are shown. B) Wound healing assays were performed on EV-231 and IRF5-231 cells. Representative pictures are shown from 0, 6 and 48 hours after scratch. Arrows point to a visible wound still present in the IRF5-231 plate at 48 hours post-scratch. Graphical representation of data from the 6 hour time point is shown on the right from at least 3 independent experiments performed in duplicate. C) Representative pictures from the matrigel evasion assay are shown. The left-most panel shows EV-231 cells suspended in the matrigel drop at time 0 hours (hr); middle panel shows EV-231 cells escaping the matrigel drop at 72 hrs; right-most panel shows IRF5-231 cells unable to escape the matrigel drop at 72 hrs. Arrows indicate the matrigel drop border. Graphical representation of data from the 72 hr time point is shown on the right. Data are from at least 3 independent experiments performed in duplicate. *p ≤ 0.05, **p ≤ 0.01.