IRF5 is a major regulator of mammary epithelial cell migration. A) Graphical representation of data from EV-231 and IRF5-231 MTT assays. B) Graphical representation of 231 cell viability at different time points post-transfection. C) Similar to B) except data shown are percentage of 231 viable cells post-transfection. No statistical difference was found in the number (A) or percentage (B) of viable cells between EV-231 and IRF5-231. D) Graphical summary of data from Boyden chamber assays measuring cell migration to serum-containing media. E) Similar to D) except data are from Boyden chamber assays measuring cell invasion through matrigel. F) Western blot analysis of endogenous IRF5 protein levels in MCF-7 cells transfected with scrambled siRNA (scrmb siRNA) or siRNA targeting IRF5 (αIRF5 siRNA). G) Similar to D) except the migration assay was performed in MCF-7 breast cancer cells transfected with either empty vector (EV), full-length IRF5 (IRF5), scrmb siRNA or αIRF5 siRNA. All data shown are from at least 3 independent experiments performed in duplicate; *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.0001.