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Figure 5 | Molecular Cancer

Figure 5

From: A conserved region within interferon regulatory factor 5 controls breast cancer cell migration through a cytoplasmic and transcription-independent mechanism

Figure 5

The inhibition of cell migration by IRF5 is non-transcriptional and occurs in the cytoplasm. A) Graphical summary of data from the dual luciferase assay measuring ISRE transactivation in the presence of full-length IRF5 or the indicated deletion mutants. Data are from 3 independent experiments performed in duplicate. B) Western blot analysis of cytoplasmic (C) and nuclear (N) extracts from 231 cells transiently transfected with the indicated plasmids. α-tubulin was used as a control for extract purity. C) Schematic representation of full-length IRF5 with a mutated 5′ NLS (mNLS). D) Graphical summary of data from the migration assay comparing EV migratory function to full-length IRF5 and the mNLS mutant in 231 cells. Data are from at least 3 independent experiments performed in duplicate; ***p ≤ 0.0001.

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