IRF5 is a global regulator of epithelial cell migration. A) Western blot analysis of endogenous and ectopic IRF5 expression in PANC1, HT29 and Huh 7.5 epithelial carcinoma cell lines. B) Graphical summary of data from the migration assay comparing EV migratory function to full-length IRF5 in PANC1 pancreatic carcinoma cells. Left panel shows fold-change; right panel shows the total number (#) of migrated cells. Data are from 3 independent experiments performed in duplicate. C) Same as in B) except migration of HT29 colorectal carcinoma cells was examined. D) Same as B) & C) except migration of Huh7.5 hepatic carcinoma cells was examined. Fold change is shown. *p ≤ 0.05, **p ≤ 0.01.