Figure 1

CCR7 promotes expression and secretion of lymphangiogenic factor VEGF-C. (A) Western blots (B) real-time PCR and (C) quantitative real-time PCR validating CCR7 knockdown in MDA-MB-231 cells versus control shRNA. (D) Western blot, (E) real time PCR and (F) quantitative real-time PCR analysis outlining decreased VEGF-C expression in MDA-MB-231 CCR7 shRNA cells versus control shRNA. (G) ELISA demonstrates that VEGF-C protein concentration in conditioned media from CCL21 treated/untreated CCR7-KD cells decreased significantly compared to parental or scrambled MDA-MB-231 cells. (H) Western blot and (I) quantitative real-time PCR validating CCL21 overexpression in MCF-7 cells. (J) Western blot, (K) quantitative PCR outlining increased VEGF-C expression in MCF-7 CCL21 cells versus control mock. (L) VEGF-C protein concentration in conditioned media from MCF-7 CCL21 and control cells as measured by ELISA. In (A-K) data are represented as mean ± SD (n = 4), except (G) and (L) where data are represented as mean ± SD (n = 3). (*) indicates statistical significant differences (p < 0.005).