Subcutaneous OX86 vaccination in mice. a. Various vaccinations to induce effector T cells, determined by flow cytometry (mean ± SEM, *P < 0.05). b. IFN-γ production determined by ELISA (mean ± SEM, *P < 0.05). Lymphocytes from vaccinated mice were stimulated with irradiated GL261 cells, and the secreted IFN-γ was measured in the conditioned medium. c. Images and weights of spleens isolated from mice 3 days after vaccination with OX86 s.c. or IgG s.c., administered twice with a 5-day interval (n = 5) (mean ± SEM, *P < 0.05).