Accumulation of CD4 and CD8 cells at the transplanted glioma cells after OX86 vaccination. a. Representative image of intracranial GL261 cells in mice received subcutaneous OX86 or IgG vaccination. CD4 and CD8 cells (green), DAPI (blue), apoptotic cells (brown) by TUNEL, and H&E. Scale bar, 40 μm. b. Numbers of CD4 cells, CD8 cells, and apoptotic tumor cells (under 400× magnification, mean ± SEM, *P < 0.05).