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Figure 2 | Molecular Cancer

Figure 2

From: Single live cell TGF-β signalling imaging: breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity

Figure 2

TGF-β signalling promotes cell migration. MDA-MB-231 cells were infected with Ad.CAGA-Fluc and Ad.CMV-Gluc virus then stimulated with ± TGF-β (5 ng/ml) before being seeded onto the apical chamber of transwells and a 96-well plate in triplicate for 48 h. Cells on the underneath of the transwell membrane and in the 96-well plate were lysed. A. The number of migrated cells through the transwell membrane was calculated as described in the Materials and Methods (Average ± SD). These data are representative of 3 separate experiments (*P < 0.05). B. Lysates were then assessed for Smad3 luciferase activity per cell with TGF-β stimulation (■) or without (□) and normalized with CMV-driven Gluc activity (Average ± SD).

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