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Figure 3 | Molecular Cancer

Figure 3

From: Single live cell TGF-β signalling imaging: breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity

Figure 3

Live cell TGF-β signalling promotes cell migration. MDA-MB-231 cells were infected with Ad.CAGA-Td-Tom virus and stimulated with ± TGF-β (5 ng/ml) before being seeded in the apical chamber of transwells and a 24-well plate in triplicate for 48 h. Cells on the underneath of the transwell membrane and in the 24-well plate were fixed, permeabilised and stained with DAPI. A. Images were taken of the cells underneath the membrane (migrated cells) and the 24-well plate (non-migrated cells) using a fluorescent microscope visualizing Smad3 active cells (red) and nuclear staining (blue). These data are representative of 3 separate experiments. B. The relative pixel intensity of Smad3 activity was quantified using imageJ software and presented as the average of 3 randomly chosen fields ± SD; (n = 3; *P < 0.05).

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Molecular Cancer

ISSN: 1476-4598