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Figure 4 | Molecular Cancer

Figure 4

From: Single live cell TGF-β signalling imaging: breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity

Figure 4

TGF-β Signalling within live individual tumour cells promotes cell motility. A. MDA-MB-231 cells were infected with Ad.CAGA-Td-Tom virus and stimulated with ± TGF-β (5 ng/ml). 24 h later, cells were tracked for 10 h and imaged every 4 min. Images were quantified with the use of Metamorph software, to track the distance moved by individual cells with (i) undetectable Smad3 activity and (ii) Smad3 activity following infection with Ad.CAGA-Td-Tom virus and TGF-β stimulation (n = 10). B. Average Speed (um/min) of each group of 10 cells (*P < 0.05). C. Cells displaying Smad3 activity were divided into 3 categories based on the intensity (low, medium and high) of Smad3 activity. Data represent the average distance travelled over time from each group (n = 7).

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Molecular Cancer

ISSN: 1476-4598