Figure 3

miR-663 attenuates pancreatic cancer cell proliferation. A. The expression of miR-663 was upregulated in the cells trasfected with miR-633 agomir. The expression of miR-663 was measured by RT-PCR. B. A statistically significant decrease in PANC-1 cell proliferation in the miR-663 group was observed compared to the Blank and NC groups. Effect of miR-663 on cell proliferation was measured by CCK-8 assay. C. A statistically significant decrease in AsPC-1 cell proliferation in the miR-663 group was observed compared to the Blank and NC groups. Effect of miR-663 on cell proliferation was measured by CCK-8 assay. D. miR-663 transfection of PANC-1 reduced growth of colonies. Colony formation in soft agar after 14 days using PANC-1 exposed to miR-663. Quantification of clone number per 10× microscopic field for cells exposed to miR-663 in duplicate experiments performed with triplicate wells. Compared to the Blank and NC groups, a significant reduction (*P < 0.05) in colony forming potential was observed after treatment with miR-663 as assessed by counting the number of clones. E. miR-663 transfection of AsPC-1 reduced growth of colonies. Colony formation in soft agar after 14 days using AsPC-1 exposed to miR-663. Quantification of clone number per 10× microscopic field for cells exposed to miR-663 in duplicate experiments performed with triplicate wells. Compared to the Blank and NC groups, a significant reduction (*P < 0.05) in colony forming potential was observed after treatment with miR-663 as assessed by counting the number of clones.