Figure 4

miR-663 attenuates pancreatic cancer cell invasiveness. A. miR-663 attenuates pancreatic cancer PANC-1 cell invasiveness. Invasive ability of PANC-1 after transfection was assessed using Transwell assays. The number of invasive miR-663-transfected PANC-1 cells was significantly lower than in the Blank and NC groups. *P < 0.05 compared to the control group. B. miR-663 attenuates pancreatic cancer AsPC-1 cell invasiveness. Invasive ability of AsPC-1 after transfection was assessed using transwell assays. The number of invasive miR-663-transfected AsPC-1cells was significantly lower than in the Blank and NC groups. *P < 0.05 compared to the control group. C. miR-663 attenuates protein expression of MMP9, Akt, pAkt and eEF1A2 in PANC-1 cells. Protein expression was measured by Western blot assay. miR-663 significantly attenuates protein expression of MMP9 , Akt and pAkt in PANC-1 cells compared to the Blank and NC groups. β-actin was used as a reference. D. miR-663 attenuates protein expression of MMP9, Akt, pAkt and eEF1A2 in AsPC-1 cells. Protein expression was measured by Western blot assay. miR-663 significantly attenuates protein expression of MMP9, Akt and pAkt in AsPC-1 cells compared to the Blank and NC groups. β-actin was used as a reference.