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Figure 5 | Molecular Cancer

Figure 5

From: RETRACTED ARTICLE: miR-663 attenuates tumor growth and invasiveness by targeting eEF1A2 in pancreatic cancer

Figure 5

miR-663 induces cell apoptosis in PANC-1 and AsPC-1 cells. A. miR-663 induces PANC-1cell apoptosis. The apoptosis cells of PANC-1 were assessed using flow cytometry assay. The number of apoptosis miR-663-transfected PANC-1 cells was significantly higher than in the Blank and NC groups. *P < 0.05 compared to the control group. B. miR-663 induces AsPC-1 cell apoptosis. The apoptosis cells of AsPC-1 were assessed using flow cytometry assay. The number of apoptosis miR-663-transfected AsPC-1 cells was significantly higher than in the Blank and NC groups. *P < 0.05 compared to the control group. C. Results from Hoechst 33342 staining assay showed that transfection with the miR-663 agomir led to a significant increase in PANC-1 cell apoptosis compared to the Blank and NC groups (P < 0.05). D. Results from Hoechst 33342 staining assay showed that transfection with the miR-663 agomir led to a significant increase in AsPC-1 cell apoptosis compared to the Blank and NC groups (P < 0.05). E. miR-663 increased protein expression of BAD, BAX, and cleaved caspase-3 in PANC-1 cells. Protein expression was measured by Western blot assay. The protein levels of BAD, BAX and cleaved caspase-3 were enhanced in miR-663 group in PANC-1 cells compared to the Blank and NC groups. β-actin was used as a reference. F. miR-663 increased protein expression of BAD, BAX and cleaved caspase-3 in AsPC-1 cells. Protein expression was measured by Western blot assay. The protein levels of BAD, BAX and cleaved caspase-3 were enhanced in miR-663 group in AsPC-1 cells compared to the Blank and NC groups. β-actin was used as a reference.

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