eEF1A2 is the direct target of miR-663 in PANC-1 and AsPC-1 cells. A. The putative miR-663 binding sequences for the eEF1A2 3′ UTRs. The 3′ untranslated region (3′UTR) of eEF1A2 contains a seed region for miR-663. B. Western blot analysed eEF1A2 expression in transfected cells. Transfection of miR-663 agomir resulted in significant reduction of eEF1A2 protein expression by western blot in PANC-1 and AsPC-1 cells. β-actin was used as a reference. C. miR-663 significantly decreased the luciferase activity of wild type in PANC-1. D. miR-663 significantly decreased the luciferase activity of wild type in AsPC-1 cells. (*P < 0.05).