Figure 8

Expression of eEF1A2 restored the pro-apoptotic function and anti-migration of miR-663. A. eEF1A2 protein level was detected by western blot assay. Cells were transfected with pcDNA3.1- eEF1A2 (not including 3′UTR) or (and) miR-663. Western blot assay showed that transfection of miR-663 agomir inhibited the expression of eEF1A2. Co-transfection of pcDNA3.1- eEF1A2 and miR-663 abrogated the effects of miR-663 on eEF1A2 expression. β-actin was used as a reference. B. Expression of eEF1A2 restored the pro-apoptotic function of miR-663. Cells were transfected with pcDNA3.1- eEF1A2 (not including 3′ UTR) or (and) miR-663. The cell apoptosis were assessed using flow cytometry assay. Co-transfected cells with pcDNA3.1- eEF1A2 and miR-663, the expression of eEF1A2 lacking the 3′ UTR sequence was found to restore the pro-apoptotic functions of miR-663. C. Expression of eEF1A2 restored the anti-migration function of miR-663. Cells were transfected with pcDNA3.1- eEF1A2 (not including 3′ UTR) or (and) miR-663. Invasive ability was assessed using transwell assays. Co-transfected cells with pcDNA3.1- eEF1A2 and miR-663, the expression of eEF1A2 lacking the 3′ UTR sequence was found to restore the anti-migration functions of miR-663.