Figure 9

eEF1A2 silencing and miR-663 overexpression exert anti-proliferative, anti-invasive and pro-apoptotic effects on pancreatic cancer cells. A. eEF1A2 silencing and miR-663 overexpression inhibited the proliferation. CCK8 array was used to assess PANC-1 proliferation. B. eEF1A2 silencing and miR-663 overexpression reduced growth of colonies of PANC-1 cells (*P < 0.05). Colony formation assay was used. C. eEF1A2 silencing and miR-663 overexpression suppressed PANC-1 cell invasiveness. Invasive ability of PANC-1 after transfection was assessed using Transwell assays. (*P < 0.05). D. eEF1A2 silencing and miR-663 overexpression induced PANC-1cell apoptosis. The apoptosis cells of PANC-1 were assessed using flow cytometry assay. (*P < 0.05). E. Protein level was detected by western blot assay. MMP9, pAKT and eEF1A2 were significantly reduced, and that BAD, BAX and cleaved caspase-3 were significantly increased in the miR-663 overexpressing and eEF1A2 silencing cells. β-actin was used as a reference.