Relative expression of SPRY4-IT1 in breast cancer tissues and cells compared with adjacent normal tissues and normal breast epithelial cells. (A) Relative expression of SPRY4-IT1 in breast cancer tissues (T) (n = 48) compared with corresponding non-tumor tissues (N) (n = 48). SPRY4-IT1 expression was examined by qPCR and normalized to GAPDH expression and the levels in the cells of interest were compared with the expression levels inMCF-10A and other cells. The results are presented as the -ΔΔCT changes in tumor tissues relative to normal tissues. (B and C) The data are presented as the relative expression levels in tumor tissues. SPRY4-IT1 expression was significantly higher in patients with a higher pathological stage and a larger tumor size (shown as -ΔΔCT). (D) SPRY4-IT1 expression was assessed by qRT-PCR in ER(−) breast cancer tissues and ER(+) breast cancer tissues(shown as -ΔΔCT). (E) The relative SPRY4-IT1 expression levels in breast cancer cell lines (MD-MB-231, MDA-MB-435S and MCF-7) were compared with human breast epithelial cells (MCF-10A), as assessed by qRT-PCR. * P < 0.05 and **P < 0.01.