Figure 3
From: Erk2 but not Erk1 regulates crosstalk between Met and EGFR in squamous cell carcinoma cell lines
Erk2 is required for HGF-induced amphiregulin production. (A) Erk1 and Erk2 siRNA knockdown in SCC9 cells. MAD-NT CM was used as HGF source. For the Erk2 knockdown two siRNAs (Erk2#1 and Erk2#2) were used. Knockdown was verified with Erk1/2 immunoblot. (B) Erk2 siRNA knockdown in different SCC cell lines. Non-targeting siRNA was used as negative control. Amphiregulin release was measured with sandwich ELISA. Error bars indicate SEM of three independent experiments. Asterisks indicate a statistically significant decrease (p < 0.05, paired t-test). Knockdown was verified with Erk1/2 immunoblot. Tubulin served as loading control.