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Figure 1 | Molecular Cancer

Figure 1

From: Intelectin 1 suppresses the growth, invasion and metastasis of neuroblastoma cells through up-regulation of N-myc downstream regulated gene 2

Figure 1

ITLN1 facilitates the NDRG2 expression in NB cells. (A) Western blot showing the expression of NDRG2, VEGF, and MMP-9 in solvent (mock)- or recombinant ITLN1-treated SH-SY5Y (1 and 2 μg/ml, for 24 hrs) and SK-N-BE(2) (1 μg/ml, for 24 and 36 hrs) cells. (B) Western blot showing the protein levels of ITLN1 [in culture supernatant (s) and lysate], NDRG2, VEGF, and MMP-9 (in lysate) in NB cells stably transfected with empty vector (mock) or ITLN1. (C) The transcript levels of ITLN1, NDRG2, VEGF, and MMP-9 in NB cells stably transfected with mock or ITLN1 as measured by real-time quantitative RT-PCR. (D) Western blot showing the expression of ITLN1 [in culture supernatant (s) and lysate], NDRG2, VEGF, and MMP-9 (in lysate) in SH-SY5Y and SK-N-SH cells stably transfected with sh-Scb or sh-ITLN1. (E) The transcript levels of ITLN1, NDRG2, VEGF, and MMP-9 in NB cells stably transfected with sh-Scb or sh-ITLN1 as detected by real-time quantitative RT-PCR. (F) Luciferase reporter assay showing the activity of different NDRG2 promoter fragments in NB cells stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1. *P < 0.01 vs. mock or sh-Scb.

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Molecular Cancer

ISSN: 1476-4598