ITLN1 is under-expressed in NB tissues and cell lines. (A) Immunohistochemical staining showing the expression of ITLN1 and NDRG2 in tumor specimens from 42 NB cases (arrowheads, brown). Scale bars: 100 μm. (B) Western blot showing the endogenous protein levels of ITLN1 and NDRG2 in lysate from NB tissues (n = 30), NB cell lines, and normal dorsal ganglia (DG). (C) Endogenous transcript levels of ITLN1 and NDRG2 in NB tissues (n = 30), NB cell lines, and DG as detected by real-time quantitative RT-PCR. (D) Pearson’s coefficient correlation analysis for the relationship between ITLN1 and NDRG2 transcript levels in NB tissues (n = 30). (E) Kaplan–Meier survival plots of well-defined NB patients with low and high expression of ITLN1 or NDRG2, as derived from R2 microarray analysis and visualization platform (http://hgserver1.amc.nl/cgi-bin/r2/main.cgi). *P < 0.01 vs. DG.