Figure 5

YAP1 re-expression partly abrogated the inhibitory effect of miR-15a and miR-16-1 in NCI-N87 and MGC-803. (A) YAP1 re-expression increased cell proliferation compared with miR-15a or miR-16-1 alone (*, P < 0.05; **, P < 0.001). The SDs were achieved by the 575 nm absorbance readings in 6 wells of each item. (B) Monolayer colony formation assays revealed YAP1 re-expression abolished proliferation-inhibition of miR-15a and miR-16-1 partially (*, P < 0.05; **, P < 0.001). The representative colony formation pictures were shown in the bottom. The experiments was performed in triplicate wells to get SDs. (C) The suppressed cell invasion abilities was partially alleviated by YAP1 re-expression in NCI-N87 and MGC-803 cells (*, P < 0.05; **, P < 0.001). The cells were counted in 3 random vision fields of each item to get SDs.