Figure 4

Transfection of Ik-1 and MZF1 decreases the viability, proliferation, migration, and anchorage-independent colony formation of NPM-ALK ⁺ T-cell lymphoma cells. (A) Compared with EV, transfection of Ik-1 decreased the viability of the lymphoma cells at 48 h (*: P < 0.0001). (B) In a similar fashion, transfection of MZF1 decreased cellular viability (*: P < 0.0001). (C) Ik-1 decreased cellular proliferation (*: P < 0.05; **: P < 0.01). (D) Transfection of MZF1 also reduced the proliferation of these cells (*: P < 0.05; **: P < 0.01). (E) IGF-I stimulated the migration of the lymphoma cells. Whereas this effect was substantially decreased when cells were treated with IGF-I and transfected with Ik-1 or MZF1; EV failed to induce similar effects (Karpas 299, *: P < 0.0001 in IGF-I vs. IGF-I+Ik-1 and IGF-I+MZF1, †: P < 0.01 in IGF-I+EV [Ik-1] vs. IGF-I+Ik-1, ‡: P < 0.001 in IGF-I+EV [MZF1] vs. IGF-I+MZF1; SUP-M2, §: P < 0.05 in IGF-I vs. IGF-I+Ik-1, IGF-I+EV [Ik-1] vs. IGF-I+Ik-1 and IGF-I+EV [MZF1] vs. IGF-I+MZF1, †: P < 0.01 in IGF-I vs. IGF-I+MZF1; SR-786, *: P < 0.0001 in IGF-I vs. IGF-I+MZF1, IGF-I+EV [Ik-1] vs. IGF-I+Ik-1, and IGF-I+EV [MZF1] vs. IGF-I+MZF1, ‡: P < 0.001 in IGF-I vs. IGF-I+Ik-1). (F) Ik-1 abrogated anchorage-independent colony formation of the lymphoma cells at 7 days after transfection (*: P < 0.05; **: P < 0.01; ***: P < 0.001 compared with EV). The numbers of colonies are shown in the left panel, and examples of the colonies are illustrated in the right panel. (G) Similarly, MZF1 halted the lymphoma cell colony formation (*: P < 0.05; **: P < 0.01 compared with EV). The numbers of colonies are shown in the left panel, and examples of the colonies are depicted in the right panel. Results represent means ± SE of at least 3 consistent experiments.