Figure 5
From: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
IGF-IR mRNA expressed in NPM-ALK + T-cell lymphoma cells exhibits prolonged decay time compared with IGF-IR mRNA from normal human T lymphocytes. (A) The decay of IGF-IR mRNA in normal human T lymphocytes over 8 h is illustrated. The 50% level (t1/2) of IGF-IR mRNA was detected at 0.8 h. (B) In contrast with T lymphocytes, the NPM-ALK+ T-cell lymphoma cell lines expressed remarkably higher basal levels of IGF-IR mRNA, with DEL and SUP-M2 cells demonstrating the highest and lowest levels, respectively. The t1/2 for IGF-IR mRNA level was achieved after longer periods of time in the lymphoma cells than in normal T lymphocytes (SU-DHL-1: 3.7 h, Karpas 299: 3.4 h, SR-786: 2.6 h, SUP-M2: 1.9 h, DEL: 1.5 h). The mean of the t1/2 IGF-IR mRNA decay time in the lymphoma cells was 2.62 ± 0.4 h (SE). Results shown represent the means ± SE of 3 experiments.