Table 3 PCR conditions used for DNA ligation for the construction of the human IGF-IR gene promoter (∞: hold)
From: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
Temperature | Time |
|---|---|
22°C | 30 min |
20°C | 30 min |
18°C | 30 min |
16°C | 30 min |
14°C | 30 min |
12°C | 30 min |
10°C | 30 min |
8°C | 30 min |
6°C | 30 min |
4°C | 30 min |
4°C | ∞ |