Survivin was highly expressed in leukemia stem cells compared to their differentiated counterparts. (A) KG-1a cells were separated into four subpopulations, and apoptosis-related protein micro-assays were performed using R statistical programming. (B) The relative expression level of four apoptosis-related proteins (Hsp70, survivin, XIAP, and IGF-II) were determined (*
P < 0.05, **
P < 0.01). (C) Protein assay results were confirmed by western blotting. (D) LSCs and non-LSCs viability was analyzed following inhibition of survivin, Hsp70, XIAP, or IGF-II expression with siRNA in the presence of chemical agents (Ara-C, L-Asp, and Dexamethasone [Dex]). (E) Analysis of survivin expression in MOLM13 cells following fractionation for the CD34 + CD38- population. (F) Survivin mRNA expression was analyzed in CD34+/CD34- cells from AML patients (n = 56, *P < 0.05) by real-time qPCR. (G) Survivin protein expression was analyzed in paired CD34+/CD34- samples from 20 AML patients by western-blot. (H) Line graph showing a direct comparison of survivin protein levels in CD34+/CD34- cells from paired AML samples (n = 20, *
P < 0.05).