Survivin knockdown abrogated LSCs growth, induced apoptosis, decreased self-renewal, and sensitized cells to chemotherapy. (A) Analysis of LSCs viability 24 or 48 h after transfection with survivin-siRNA (*
P < 0.05). (B) Analysis of survivin protein expression, cleaved PARP, and caspase3 expression 48 h after transfection with survivin-siRNA in KG-1a and MOLM13LSCs. Non-targeting (NT)-siRNA was used as negative control. (C) Analysis of apoptosis rates in LSCs 48 h after transfection with survivin-siRNA (*
P < 0.05). NT-siRNA was used as a negative control. (D) Analysis of the self-renewal capacity of LSCs following knockdown of survivin (*
P < 0.05). NT-siRNA was used as a control. (E) Primary AML CD34+ cells (TS3 cells) were transfected with either pcDNA-survivin vectors or siRNA and treated with combined therapy (left panel) or Ara-C alone (right panel) at the indicated concentrations (*
P < 0.05). Cell viability was detected by CCK-8 assay.