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Figure 2 | Molecular Cancer

Figure 2

From: TNFα sensitizes neuroblastoma cells to FasL-, cisplatin- and etoposide-induced cell death by NF-κB-mediated expression of Fas

Figure 2

TNFα primes for FasL-induced cell death and upregulates Fas expression. A. SK-N-AS cells were left untreated or were pre-treated for 24 h with 100 ng/ml TNFα or 100 ng/ml Fc:hFasL and subsequently washed and left untreated (UT) or treated for 24 h with 100 ng/ml TNFα or 100 ng/ml Fc:hFasL. Cell viability was assessed by calcein AM staining. B. SK-N-AS cells were treated or not with 100 ng/ml TNFα for the indicated times and mRNA levels of the indicated genes were assessed by qRT-PCR. mRNA expression levels were normalized using 18S. C. Western blot of Fas expression in SK-N-AS cells treated with 100 ng/ml TNFα for the indicated times. α-Tubulin was used as loading control. ***p ≤ 0.001.

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Molecular Cancer

ISSN: 1476-4598