Figure 6

NBLs show heterogeneous Fas expression in response to TNFα treatment. A. NBL cell lines were treated with 100 ng/ml TNFα or left untreated (UT) for 24 h. Fas expression was analyzed by cytometry using a PE-conjugated Fas or isotype antibody. B. Cells were treated for 24 h with 100 ng/ml TNFα or were left untreated (UT). Protein expression levels were analyzed by Western blot. C. Cell death assay by Hoechst staining in the indicated cell lines pre-treated with 100 ng/ml TNFα for 24 h and treated for another 24 h with 100 ng/ml Fc:hFasL, or 1 ng/ml Fc:hFasL for Tet21N cells. **p ≤ 0.01; ***p ≤ 0.001.