Figure 1

Effect of global CaSR knock-down in vivo on genes modulating EMT/stemness in the colon. Colons sampled from CaSR ^−/−/PTH ^−/− (DKO) and CaSR ^+/+/PTH ^−/− (control) mice were investigated for mRNA expression of the mesenchymal markers αSma (A), Fsp1(B), Snail2 (C), Twist2 (D), Vimentin (E), Zeb1 (F), and the pluripotency markers Nanog (G) and Stella (H). Dots indicate individual data points, and the line represents median. Statistical significance was calculated using t test. n = 9 animals/group, *p < 0.05, **p < 0.01, ***p < 0.001.