Ectopic CaSR prevents nuclear β-catenin translocation in HT29 colon cancer cells. (A) HT29 cells overexpressing the CaSR (HT29CaSR) show reduced β-catenin nuclear translocation as assessed by western blot and (B) by quantification of β-catenin signal normalized to house-keeping genes (Lamin C: nuclear fraction and α-Tubulin: cytosolic fraction). (C-F) HT29CaSR cells show increased mRNA expression of GSK-3β, of the differentiation markers CDX2 and Villin, and reduced levels of the proliferation marker Cyclin D1 compared with HT29EMP cells. Data represent mean ± SEM of three independent experiments. Statistical significance was calculated using t test. *p < 0.05, **p < 0.01, ***p < 0.001.