Ectopic CaSR prevents induction of mRNA expression of EMT markers in HT29 colon cancer cells. HT29CaSR cells (grey bars) show downregulation in mRNA expression of mesenchymal markers αSMA, FOXC2, SNAI1, TWIST2, Vimentin and Zeb1 compared with HT29EMP cells (white bars). Treatment with EMT promoting cocktail, further induced mesenchymal transition in HT29EMP cells (white striped bars), which was blocked by ectopic expression of CaSR (HT29CaSR, grey striped bars). Data represent mean ± SEM of three independent experiments. Statistical significance was determined by ANOVA followed by Tukey’s post-test. Asterisks above bars indicate all significant changes. *p < 0.05, **p < 0.01, ***p < 0.001.