Ectopic CaSR prevents induction of protein expression of EMT markers in HT29 colon cancer cells. Treatment with the EMT promoting cocktail induced protein expression of mesenchymal markers, αSMA and Vimentin only in HT29EMP cells, which was blocked by ectopic expression of CaSR (HT29CaSR). In HT29CaSR cells, the upregulated E-Cadherin expression was downregulated upon treatment with the EMT promoting cocktail but remained higher than in HT29EMP cells. The merged images (red or white channels for the indicated markers and blue for DAPI) are shown. Scale bar: 20 μm.