Figure 3

Influence of DCA on mitochondrial respiration and glycolysis. (A) OCR in A549 and A549/Taxol cells treated with 0 or 10 mM DCA for 24 h with glucose as carbon source. (B) Release of ¹⁴C-CO₂ by A549 and A549/Taxol cells treated with 0 or 10 mM DCA for 24 h. (C) OCR in A549 and A549/Taxol cells treated with 0 or 10 mM DCA for 24 h with glutamine as carbon source. (D) Influence of DCA on ECAR in A549 and A549/Taxol cells. (E) Influence of DCA on glucose uptake in A549 and A549/Taxol cells incubated in DMEM with 18 F-FDG for 1 h. Uptake of radioactivity was measured with a well gamma counter. (F) Effect of 3BrPA on ATP generation by glycolysis in A549, A549/Taxol and A549/MD cells. (G) Influence of DCA on ATP production after treatment with 0 or 10 mM DCA for 24 h. *P < 0.05. Data are mean ± SEM of three independent experiments.