Figure 5

DCA increases citrate accumulation in cells with mitochondrial injury. (A) Effects of a 3-h DCA treatment on PDH activity in A549 and A549/Taxol cells. (B) Influence of DCA on citrate accumulation measured by GC. (C) Influence of DCA on PFK activity by PFK colorimetric assay. (D) Intracellular citrate levels under normoxia in various cell lines and hypoxic conditions in A549 cell lines treated with 0 or 10 mM DCA for 24 h. (E) Comparative analysis of PDK2 expression in control and PDK2-siRNA cell lines determined by western blotting (48 h post-transfection). (F) Viability of A549 and A549/Taxol cells treated with PDK2 siRNA. (G) Influence of PDK2 siRNA on citrate accumulation. (H) Glucose uptake in control and PDK2-siRNA cells. *P < 0.05. Data are mean ± SEM of three independent experiments.