Figure 5

PinX1 inhibits breast cancer cells metastasis in vivo. (A) Left panel, Western blotting of PinX1 in there groups of Ctrl-MDA-MB-231 cell lines, PinX1^OE-MDA-MB-231 cell lines and PinX1^KD-MDA-MB-231 cell lines, which was selected with puromycin for 2 weeks after lentivirus infection. Right panel, without puromycin selection for 2 months, PinX1 expression levels were not changed in MDA-MB-231 stable cell lines. (B) H&E staining sections of lungs 2 months after injection of PinX1^KD-MDA-MB-231 cell lines in BALB/c nude mouse through tail vein. Left panel, magnification × 100; right panel, magnification × 400. (C) Representative images of 10% buffered formalin fixed lungs with metastatic nodules 2 months after injection of Ctrl, PinX1^OE and PinX1^KD MDA-MB-231 stable cell lines. Arrows indicate metastatic nodules. (D) The number of lung metastatic nodules was counted under a dissecting microscope. Compared with the PinX1^OE group, a statistically dramatic increase in the number of the lung metastases was seen in PinX1^KD group, and these two groups also had significant diversity compared with Ctrl group respectively. Data are displayed with means ± SD from 10 mice in each group. ***, P < 0.001.