Figure 3

Increased tumor incidence in aged TGFBI Tg but not TGFBI KO mice. (A) TGFBI overexpression in TGFBI Tg mice by immunohistochemistry. Organs from WT and Tg mice were cryosectioned and stained with anti-TGFBI Ab and signals were revealed by Anti-mouse/rabbit Universal Immunohistochemistry Detection Kit (Proteintech Group). (B) Tumor incidence in aged TGFBI Tg mice. Tg and WT mice were kept under specific pathogen-free conditions. They were sacrificed and autopsied starting from age 16 months if one of the following conditions was met: 1) palpable lumps in abdomen; 2) loss of more than 20% body weight; 3) death from unknown causes; 4) reached age 22 months. Percentages of mice with tumor masses upon visual inspection are reported. The data were analyzed by 2-tailed Student’s t tests. P-value is indicated. (C) Tumor masses in aged TGFBI Tg mice. Arrows indicate tumor masses in 2 aged TGFBI Tg mice. (D) Histology of tumors in aged TGFBI Tg mice. Upper panel: hepatocellular carcinoma; middle panel: gastric adenoma, arrows indicate tumorous cells; lower panel: lymphoma-like tumor. (E) Tumor incidence in aged TGFBI KO mice. Tumor incidence in aged TGFBI KO and WT mice was determined as described in A. The data were analyzed by 2-tailed Student’s t test. P-value is indicated.