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Figure 5 | Molecular Cancer

Figure 5

From: The role of TGFBI (βig-H3) in gastrointestinal tract tumorigenesis

Figure 5

TGFBI promotes liver cell survival and proliferation after irradiation. (A) Aml-12 liver cells overexpressed TGFBI after stable transfection with the TGFBI-expressing construct pCEP4-TGFBI. TGFBI mRNA levels of transfected Aml-12 liver cells were measured by RT-qPCR. β-actin mRNA levels served as internal controls, and data are expressed as ratios of TGFBI versus β-actin signals (left panel). The supernatants of these cells were quantified by ELISA in duplicate for TGFBI protein levels; regular culture medium was included as an additional control (right panel). Means ± SD of representative experiments are shown. (B) TGFBI promotes post-irradiation survival of liver cells carrying DNA damage. TGFBI-overexpressing Aml-12 and control cells were irradiated at 20 Gy and cultured for 20 h. They were then stained with Abs against γH2AX and caspase-3, and analyzed by flow cytometry. The percentages of caspase-3-positive cells among γH2AX-positive cells are presented (left), and the percentages of 3 independent experiments are summarized in the bar graph (right), with p-value indicated (2-tailed Student’s t test). (C) TGFBI promotes post-irradiation proliferation of liver cells. TGFBI-overexpressing Aml-12 and control cells after irradiation were cultured in complete DMEM/F12 medium containing 10% FCS for 40 h. 3H-thymidine was added 16 h before the cells were harvested. Thymidine uptake of both irradiated and non-irradiated cells in a representative experiment is shown on the left. Post-irradiation proliferation indices of 3 independent experiments are summarized in a bar graph on the right with p-value indicated (2-tailed Student’s t test).

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