Bax inhibition reduces SapC-DOPS-induced apoptosis. A) MTT assay in control peptide-treated (empty bar) and V5 peptide-treated (Bax-inhibited; filled bars) SK-N-SH cells following SapC-DOPS treatment. B) ΔΨM measured by JC-1 assay in SK-N-SH cells following SapC-DOPS treatment for 24 h. C) Apoptotic protein expression in control-peptide and Bax-V5-peptide pre-treated SK-N-SH cells following 50 μM SapC-DOPS treatment for 24 h. D) Changes in Smac expression in cytosolic and mitochondrial extracts of Bax-inhibited SK-N-SH cells treated with 50 μM SapC-DOPS. *P < 0.05, **P < 0.001. Points, mean of three to five experiments; bars, SE. Western blots are representative of three independent experiments.