Figure 5
From: MicroRNA-31 controls G protein alpha-13 (GNA13) expression and cell invasion in breast cancer cells
miR-31 inhibits GNA13 expression and cancer cell invasion. Altering miR-31 expression or activity impacts GNA13 mRNA and protein expression in MDA-MB-231 and MCF-10a cells. (A) GNA13 mRNA expression is reported relative to premiR-control or antimiR-control treated cells, respectively. (B) Immunoblot analysis of GNA13 and tubulin expression following treatment with premiRs or antimiRs as indicated. (C) miR-31 levels in MDA-MB-231 and MCF-10a cells following treatment with premir-31 and antimir-31, respectively. Values reported are relative to miR-31 levels in MDA-MB-231 cells treated with premiR-control. (D) PremiR-31 transfection inhibits invasion of MDA-MB-231 breast cancer cells. Invasion is reported as fold change relative to invasion of premiR-control + pMSCV-vector transfected cells. (*, p < 0.05). (E) Immunoblot analysis of GNA13 and tubulin levels in the MDA-MB-231 cells from (D).