Figure 5
From: MiR-377 targets E2F3 and alters the NF-kB signaling pathway through MAP3K7 in malignant melanoma
miR-377 effect on the 3′UTR of selected potential targets. 293 T cells were co-transfected with control empty psiCHEK-II (vector), or with a vector containing a part of the 3′UTR (containing the putative binding site for miR-377) of the MAP3K7 or E2F3, or KRAS, or CDK6 genes, attached to luciferase cDNA, concomitant with a miR-377 expressing vector at different concentrations (0.5 or 1 μg). The results are presented as the ratio of expression of renilla/luciferase that was normalized relative to control-transfected cells for each vector. Data is represented as mean ± SEM from three independent experiments. *signifies p < 0.02.