Figure 2

The role of FOXM1 in regulating its target genes. (A) Heatmap depicting mRNA expression levels of FOXM1 target genes in oesophageal adenocarcinoma samples. Samples were ranked according to the expression of FOXM1 across 78 adenocarcinoma samples [4] and genes ranked according to their similarity to FOXM1 expression across all samples (Pearson’s correlation coefficients >0.7 are shown). Dark squares shown above each gene symbol indicate that the gene was in the OE33-U2OS “shared” FOXM1 target gene dataset. (B) Nanostring nCounter gene expression analysis of mRNA levels of FOXM1 target genes in OE33 cells following transient transfection with siRNA directed against FOXM1 (siFOXM1, blue bars), for targets shared between OE33 and U2OS cells or specifically found in OE33 cells. The mRNA count was normalised by the geometric mean of ALAS1, GAPDH and HMBS expression. The mean count relative to control cells (siNTC, taken as 1, grey bars) of at least three biological replicates is shown. Error bars indicate the standard deviation. Statistical significance is indicated (P-values: ** <0.01 and * <0.05).